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A8792
Sigma
Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| MDL number: | MFCD00162456 |
Description
| Preparation Note | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Physical form | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| application(s) | direct ELISA: 1:40,000 |
| dot blot: 1:6,000 | |
| immunoblotting (chemiluminescent): 1:80,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150 | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Andrews, N., et al., Time resolved fluorometric immunoassay, using europium labelled antihuman IgG, for the detection of human tetanus antitoxin in serum J. Clin. Pathol. 54, 812-815, (2001) Abstract |
| Andrews, N., et al., Immunity to diphtheria and tetanus in England and Wales. Vaccine 19, 167-173, (2000) Abstract | |
| Bulmer, J., et al., Maternal HIV infection and placental malaria reduce transplacental antibody transfer and tetanus antibody levels in newborns in Kenya. J. Infect. Dis. 196, 550-557, (2007) Abstract |
